Laboratory study demonstrates superior ultrastructural preservation in Bacillus subtilis dormant spores, highlighting an effective triple-fixation protocol for thick spore coats.
A triple-fixation method with a sequential application of 5% glutaraldehyde, 1% osmium tetroxide, and 2% potassium permanganate gave superior preservation of the ultrastructure of Bacillus subtilis dormant spores with a thick spore coat.
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Kozuka et al. (1983) studied this question.
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