According to prevalent agronomic practices for cultivation of Brassica genotypes, N is applied in split doses, while S is applied as a basal dose. This may create imbalance in the supply of these nutrients during the growth and development of the crop because metabolism of N and that of S are closely linked and play a central role in protein synthesis. The requirement of one depends on the supply of the other, and the imbalance in their supply causes a reduction in the yield because of reduced uptake and assimilation of the two nutrients. In the present investigation, therefore, S was applied in split doses, along with N, to study its effect on growth and yield attributes of Brassica juncea (L.) Czern. and Coss. (V 1 ) and Brassica campestris L. (V 2 ). In the experiment, conducted in the field, 40 kg S ha −1 as CaSO 4 (gypsum) was applied either in a single basal application (S 1 ) or in two (S 2 ) or three (S 3 ) split applications; and 100 kg N ha −1 as urea was applied either in two (N 2 ) or three (N 3 ) splits. Biomass accumulation, leaf-area index (LAI), leaf-area duration (LAD), crop growth rate (CGR) and photosynthesis in the leaves were determined at various phenological stages. Split application of S and N (S 2 N 2 or S 3 N 3 ) resulted in significant improvement in growth and yield of both the genotypes compared with the application of S in a single basal application and N in two splits (S 1 N 2 ). Genetic variability was observed between the two genotypes in response to split application of S and N. V 1 responded better when S and N was applied in two split doses (S 2 N 2 ) than when it was applied as S 1 N 2 or S 3 N 3 This S 2 N 2 treatment resulted in 40.0, 39.7, 35.5, 48.2 and 18.1% enhancement in biomass accumulation, LAI, LAD, CGR and photosynthetic rate, respectively in comparison with S 1 N 2 . Seed yield, biological yield and harvest index were improved by 38.3, 26.3 and 9.5%, respectively, by S 2 N 2 over the results obtained with S 1 N 2 . In the case of V 2 , three split applications of S and N (S 3 N 3 ) resulted in maximum growth and yield. Increases in biomass accumulation, LAI, LAD, CGR and photosynthetic rate due to application of S 3 N 3 were 48.4, 81.3, 77.9, 101.1 and 28.6% respectively, over the results of S 1 N 2 . Seed yield, biological yield and harvest index improved by 41.3, 26.9 and 11.6% respectively, with this treatment.On the basis of results obtained in this study, it can be concluded that S must be applied in split doses for optimum growth and yield of Brassica genotypes. The variability in response of these genotypes to split application of S and N was due to differences in flowering time, as V 1 flowered earlier (just after the application of the second dose of S and N) than V 1 (just after the application of the third dose of S and N).
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Ahmad et al. (1999) studied this question.
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