A Cytochrome c ‐Containing Periplasmic Nitrate Reductase in the Acetogen Sporomusa ovata

This is the authors' abstract. We don't add key points for this paper.

were used simultaneously. Genome analyses revealed a nitrate reduction island with genes encoding a periplasmic cytochrome c-containing nitrate reductase, nitrite reductase, cytochrome c and heme biosynthesis. The expression of this nitrate reduction island was strongly induced by the presence of nitrate. Enzyme assays demonstrated membrane-bound nitrate and nitrite reductase activities exclusively in nitrate-grown cells. Heme peroxidase staining confirmed the presence of cytochromes in nitrate-grown cells. In sum, nitrate reduction to ammonium is catalysed by a membrane-bound electron transport chain involving cytochromes and potentially coupled to energy conservation in S. ovata.