The quantification of synthetic corticosteroids using isotope dilution gas chromatography negative chemical ionization mass spectrometry

Prednisolone, dexamethasone and betamethasone were labelled with deuterium via a simple synthetic procedure and used as internal standards in the gas chromatographic/mass spectrometric analysis of the corresponding undeuterated compounds. The mass spectrometer was used in the negative chemical ionization mode, which gave fragmentation of the methoxime trimethylsilyl ether derivatives favourable for their quantification. The method was applicable to the quantification of synthetic corticosteroids contained in human aqueous humour in the 0.1-10-ng range.