Rate constants for reaction of hydroxyl radicals with Tris, Tricine and Hepes buffers

Rate constants for the reactions of three commonly used organic buffers and hydroxyl radicals were measured using steady‐state competition kinetics with thymine. For Hepes (4‐(2‐hydroxyethyl)‐1‐piperazine‐ethanesulphonic acid), Tricine ( N ‐[2‐hydroxy‐1,1‐bis(hydroxymethyl)ethyl]glycine and Tris (2‐amino‐2‐hydroxymethylpropane‐1,3‐diol) the rate constants were 5.1×10 9 , 1.6×10 9 and 1.1 × 10 9 1·mol −1 ·s −1 , respectively.