DVB-cross-link latex microspheres to uniformly host Eu(Ⅲ) chelates with moderate swelling capacity for high performance quantitative LFIA strips

Key Points

• The aim is to enhance the performance of lateral flow immunoassay by developing polystyrene microspheres that can uniformly host europium chelates.
• Developed DVB-cross-link polystyrene microspheres for optimal europium chelate hosting.
• Evaluated swelling capacity to ensure moderate absorption without excessive concentration quenching.
• Tested performance in lateral flow immunoassays to assess biodetection efficiency.
• The modified microspheres showed a consistent uniformity in hosting europium chelates.
• A moderate swelling capacity was achieved, leading to improved fluorescence stability.
• Higher detection sensitivity was observed compared to conventional microspheres.