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May 8, 2026Journal of Proteome Research

Offline Two-Stage SEC and LC-MS/MS for Comprehensive Characterization of Yeast Ribosomal Populations

Andrew J. Spiteri Joshua J. Hamey Marc Wilkins

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Key Points

This research aims to characterize yeast ribosomal populations and investigate their functional changes during polysome collapse.
Utilized offline two-stage size exclusion chromatography (SEC) combined with LC-MS/MS for analysis.
Focused on detecting changes in ribosome composition during functional perturbations.
Employs polysome profiling to assess the interactions and compositions of ribosomal populations.
Demonstrated rapid polysome collapse in yeast under knockout conditions.
Detected significant alterations in polysome profiling ratios linked to ribosomal composition changes.

Implication

Shows changes in ribosomal populations during polysome collapse, indicating effects on function.

Abstract

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Cite This Study

Spiteri et al. (2026) studied this question.

synapsesocial.com/papers/6a002222c8f74e3340f9d178 https://doi.org/10.1021/acs.jproteome.5c00945

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Keep reading

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