LncRNA PVT1 promotes ovarian cancer progression by silencing miR-214

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OBJECTIVE: Emerging evidence indicates that long non-coding RNAs (lncRNAs) are critical in carcinogenesis and progression of ovarian cancer. This study aimed to explore the functions and molecular mechanisms of plasmacytoma variant translocation I (PVT1) in ovarian cancer. METHODS: PVT1 and miR-214 were detected by qRT-PCR assays in ovarian cancer tissues and cells. The cell proliferation, migration, and invasion abilities were detected by cell functional experiments, respectively. Western blot assay was performed to detect epithelial-mesenchymal transition (EMT) markers. MiR-214 expression regulated by PVT1 was studied by RNA immunoprecipitation (RIP) and chromatin immunoprecipitation (ChIP) assays. The promotion of ovarian cancer progression by PVT1 involved in regulation of the epithelial-mesenchymal transition process and PVT1 interaction with EZH2 represses miR-214 expression in ovarian cancer cells. CONCLUSIONS: PVT1 plays an important role in ovarian cancer tumorigenesis, which might be as a novel diagnostic marker and therapeutic target for ovarian cancer.