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BACKGROUND: sp. in stool samples after deoxyribonucleic acid (DNA) extraction. Protocols of stool pretreatment have been proposed to optimize oocysts' DNA extraction. Among them, mechanical grinding was reported to improve the performance of Cryptosporidium oocysts' DNA extraction. METHODS: oocyst' DNA extraction, before amplification using the same real-time PCR method targeting. CONCLUSIONS: DNA amplification methods.
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Valeix et al. (2020) studied this question.
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