Engineered methanotrophic syntrophy in photogranule communities removes dissolved methane

. The overall COD balance closed at around 91%. Small photogranules removed methane more efficiently than larger photogranule, likely because of a more favorable surface to volume ratio of the biomass. MiSeq amplicon sequencing of 16S and 23S rRNA revealed a potential syntrophic chain between methanotrophs, non-methanotrophic methylotrophs and filamentous cyanobacteria. The community composition between individual photogranules varied considerably, suggesting cross-feeding between photogranules of different community composition. Methanotrophic photogranules may be a viable option for dissolved methane removal as anaerobic effluent post-treatment.