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May 11, 2026VirologyOpen Access

A Two-Component RUBY Reporter System to Assess Plant Virus Infection

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Authors

HNHaritha NunnaJLJudith LinMKMichael Koretsky

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Overview

Randomized trial shows non-invasive visualization of virus infection in plants, indicating a new monitoring technique.

Key Points

  • The research aims to enhance monitoring of plant virus infections using a RUBY reporter system.
  • Engineered L-DOPA 4,5-dioxygenase into TMV and TuMV vectors.
  • Transformed CYP76AD1 and glucosyltransferase for expression in Nicotiana benthamiana.
  • Inoculated transgenic plants with TMV and TuMV vectors expressing DODA.
  • Visible red tissues produced in transgenic plants upon inoculation with virus vectors.
  • Demonstrated direct visualization of virus infection without destructive sampling.
  • Established a robust non-invasive method for tracking virus movement.

Cite This Study

Nunna et al. (2026) studied this question.

synapsesocial.com/papers/6a0171983a9f334c28271b33https://doi.org/10.1016/j.virol.2026.110944
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