Effects of ionic strength and pH on the adsorption equilibria of lysozyme on ion exchangers

The effects of ionic strength and pH on adsorption of lysozyme to three strong cation exchangers have been studied. Adsorption equilibrium data obtained using the batch techniques corresponded well to the Langmuir isotherm. Ionic strength had a considerable influence on the isotherms. In all cases, the maximum binding capacity of the three exchangers decreased while the apparent dissociation constant increased with increasing ionic strength. The maximum binding capacity also decreased while the apparent dissociation constant was not significantly affected by an increase in pH. It was found that the three exchangers exhibited different levels of binding capacity under identical solution conditions. This was shown to be caused by variation in the arrangement and distribution of charged groups. However, the ion‐exchange matrix had little effect on the apparent dissociation constant.