Masked fumonisins in processed food: co-occurrence of hidden and bound forms and their stability under digestive conditions

Fumonisins can occur in foodstuffs as free forms (prent, partially hydrolysed, or totally hydrolysed), as covalently bound fumonisins (bound fumonisins) and as non-covalently bound fumonisins (hidden fumonisins). The formation of several covalently bound fumonisin B 1 conjugates upon reaction with sugars and/or amino acids has been reported in the literature so far, but occurrence data are still very poor. Since very little is known about the relevance of both hidden and bound fumonisins in processed products, the present study aimed to evaluate the occurrence of both masked forms in maize-based food products and to study their stability under digestive conditions. The behaviour of covalently bound fumonisin B 1 conjugates upon in vitro digestion was evaluated, demonstrating their stability under these conditions. In addition, the co-occurrence of hidden and bound fumonisins in maize-based food products was investigated by application of both alkaline hydrolysis and simulated in vitro digestion assay. The experiments clearly showed that bound fumonisins were rather stable under the applied conditions, whereas hidden fumonisins released their parent forms.