Wildlife serves as a natural reservoir for emerging viruses. Although virome studies of bats and rodents have advanced our understanding of viral diversity, the virome characteristics and zoonotic risks of wildlife inhabiting the Qinghai-Tibet Plateau-a global biodiversity hotspot-remain poorly understood. Here, we conducted a virome survey of 22 wild animal species from the Qinghai-Tibet Plateau using meta-transcriptomic sequencing. We characterized the diversity and distribution of wildlife-associated viruses and found that birds tended to harbor higher viral diversity and abundance than mammals. In addition, greylag goose (Anser anser) and Himalayan vulture (Gyps himalayensis) harbored a high proportion of cross-species viruses and occupied central positions in the transmission network. In total, we identified 36 viral genera with pathogenic potential to humans or animals. Among them, several viruses, including rotavirus A (RVA) and parainfluenza virus 5 (PIV-5), pose a high risk of zoonotic transmission. Notably, the plateau vole (Neodon fuscus) carried the highest number of high-risk viruses, and the RVA strains it harbors represent a distinct early evolutionary lineage. These findings highlight the public health relevance of wildlife on the Qinghai-Tibet Plateau and emphasize the need for continued surveillance of high-risk hosts and viruses.
Zhou et al. (2026) studied this question.