Agrobacterium-mediated transient expression via root absorption in flowering Chinese cabbage

BACKGROUND: -mediated transient transformation system that utilized root absorption for research in flowering Chinese cabbage. RESULTS: = 0.3 for 3-6 h, without hyperosmotic pretreatment, and under these conditions, the peak transformation efficiency was observed at 2 and 4 d after infection. CONCLUSIONS: The transformation method developed by the present study is simple and convenient, since no special equipment is required, and since the method causes no damage, the plants can be used for subsequent experiments.