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Reactive but biologically inert: The bioorthogonal title reaction enables highly specific chemical modifications, such as lipidation, of engineered proteins containing a diphenyltetrazole group (see scheme). The cycloaddition in biological media is extremely fast (≤1 min) and tolerant of proteinaceous groups. Strongly fluorescent pyrazoline cycloadducts are generated with simple alkenes.
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Song et al. (2008) studied this question.
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