In Vitro Antifungal Activity Screening of Crude Extracts of Soil Fungi Against Plant Pathogenic Fungi

The ethyl acetate extracts of the culture of Eupenicillium parvum, Gelasinospora brevispora, Neosartoryap seudofischeri, N. quadricincta and N. multiplicata were assessed for their antifungal activity against ten economically important plant pathogenic fungi: Pythiumaphanidermatum, Phytophthorapalmivora, Alternaria sp., Fusariumoxysporum, Colletotrichumgloeosporioides, Lasiodiplodiatheobromae, Helminthosporiummaydis, Sclerotiumrolfsii and Rhizoctoniasolani,which arecausative agents of fruit and vegetable diseases. The bioassay for the antifungal activity of the fungal crude extracts was based on the dilution plate method. Although all the extracts exhibited a complete inhibition of the mycelial growth of some plant pathogenic fungi at the highest concentration tested (10,000 ppm), an interesting antifungal effect was observed for the crude extract of N. pseudofischeri KUFA 0060 against Ph. palmivora and C. capsiciat 100 ppm, as well as for N. quadricincta KUFA 0064 against Ph. palmivora and Alternaria sp. at 10 ppm. The results obtained from this screening allow us to identify new potential sources for the development of alternative fungicides.