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May 11, 2026MicrobiologyOpenOpen Access

Safety Evaluation of Weissella paramesenteroides MW‐142 Isolated From Traditional Fermented Mulberry Wine

Juan Huang Huang Y Dongmei Liu Qing‐bo Yao Jun Tang Suping Zeng Liu-Jun Liu

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Key Points

This research aims to evaluate the safety of Weissella paramesenteroides MW-142 for potential industrial use.
Conducted toxicity assessments over 14 days without signs of poisoning or mortality.
Performed hemolysis tests and biochemical assays for harmful metabolites.
Carried out genomic screening for virulence-associated genes and antibiotic resistance.
Analyzed organ morphology via histopathological examination.
No hemolytic zones were observed in hemolysis tests, indicating non-pathogenicity.
Biochemical assays showed no harmful metabolites like indole or toxins were present.
Identified 83 putative virulence-associated genes; however, most had < 50% sequence similarity with known toxins.
Only low-risk intrinsic antibiotic resistance genes were identified, with no associated mobile genetic elements.

Implication

Safety evaluation shows Weissella paramesenteroides MW-142 exhibits no hemolysis or harmful effects, indicating it may serve as a probiotic.

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Cite This Study

Huang et al. (2026) studied this question.

synapsesocial.com/papers/6a03cbfc1c527af8f1ecfbef https://doi.org/10.1002/mbo3.70302

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