Synthesis of Small Lipid Nanoparticles Using an Inkjet Mixing System Aiming to Reduce Drug Loss

Abstract An inkjet mixing system was investigated to synthesize lipid nanoparticles (LNP) by mixing lipid ethanol and aqueous saline solutions. The system was employed to help minimize drug loss. The quick mixing of the droplets enabled the synthesis of small LNP while reducing the excess amount of saline solution that would contain drugs. The system also features minimal usage of reagents. A high relative velocity of the droplets and moderate values of the impact parameter were necessary for successful mixing. Faster droplets should collide with the upper or horizontal level of slower droplets to promote mixing. A horizontal spin of the coalesced droplets was favored rather than a vertical spin.