Targeted Mutagenesis of CKX3 Homologs Enhances Seed Yield in Soybean

Summary statement This study demonstrates that CRISPR/Cas9‐mediated knockout of the cytokinin oxidase gene CKX3a in soybean simultaneously increases both seed number per plant and individual seed weight, resulting in a 52.4% yield increase—comparable to yield enhancements previously observed in Arabidopsis. The increase in seed size is attributed to enhanced cell division. These findings establish CKX3a as a promising genetic target for improving soybean yield.