Protein Salting-Out Method Applied to Genomic DNA Isolation from Fish Whole Blood

Isolation of genomic DNA is a primary molecular biology technique that been increasingly applied in the research fields of aquaculture, fisheries fish biology. There are two major isolation procedures, the organic method and the protein salting-out method. Both methods differ in manner in which partially hydrolyzed polypeptides were separated precipitated following cell lysis proteinase K treatment. The organic extraction method is based on the use organic solvents such as phenol and