Vertically-aligned nanocrown electrodes achieved a >99% success rate in obtaining intracellular access through electroporation for parallel and robust recording of cardiomyocytes.
Nanocrown electrodes enable highly reliable, parallel, and prolonged intracellular action potential recordings in cardiomyocytes, offering a robust platform for preclinical proarrhythmia drug screening.
Absolute Event Rate: 99.35% vs 93.83%
Drug-induced cardiotoxicity arises primarily when a compound alters the electrophysiological properties of cardiomyocytes. Features of intracellular action potentials (iAPs) are powerful biomarkers that predict proarrhythmic risks. In the last decade, a number of vertical nanoelectrodes have been demonstrated to achieve parallel and minimally-invasive iAP recordings. However, the large variability in success rate and signal strength have hindered nanoelectrodes from being broadly adopted for proarrhythmia drug assessment. In this work, we develop vertically-aligned nanocrown electrodes that are mechanically robust and achieve > 99% success rates in obtaining intracellular access through electroporation. We validate the accuracy of nanocrown electrode recordings by simultaneous patch clamp recording from the same cell. Finally, we demonstrate that nanocrown electrodes enable prolonged iAP recording for continual monitoring of the same cells upon the sequential addition of four incremental drug doses. Our technology development provides an advancement towards establishing an iAP screening assay for preclinical evaluation of drug-induced arrhythmogenicity.
Jahed et al. (2022) studied Cardiomyocyte electrophysiology. Nanocrown electrodes vs. Nanopillar electrodes was evaluated on Electroporation success rate. Vertically-aligned nanocrown electrodes achieved a >99% success rate in obtaining intracellular access through electroporation for parallel and robust recording of cardiomyocytes.