Integrated transcriptomic and proteomic analysis reveals inflammatory activation and blood-brain barrier disruption during meningitis-associated extraintestinal pathogenic Escherichia coli infection

experiments demonstrated RS218-induced cell death in brain endothelial cells, microglial cells, and peritoneal macrophages. Animal experiments confirmed systemic metabolic disruption, immune cell alteration, functional BBB disruption, and profound brain cytokine elevation. This integrated analysis advances our understanding of bacterial meningitis pathogenesis and identifies potential therapeutic targets.