Identification of two multifunctional P450 enzymes for biosynthesis of sesteraltererol-type sesterterpenoids

Key Points

• This research aims to identify and characterize multifunctional P450 enzymes involved in the biosynthesis of sesteraltererol-type sesterterpenoids.
• Identified a five-gene cluster related to sesteraltererol biosynthesis.
• Characterized the enzyme AasA and compared it with another known terpene synthase, AaTPS1.
• Analyzed the catalytic processes of these enzymes in producing sesterterpenoids.
• AasA catalyzes the formation of sesteraltererol, similar to AaTPS1.
• Demonstrated the involvement of other multifunctional P450 enzymes in sesterterpenoid biosynthesis.
• Showed that the five-gene cluster is essential for efficient production of sesteraltererol-type compounds.