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May 15, 2026Russian Journal of Bioorganic Chemistry

Bicelles as a Prospective Membrane Mimetic for NMR Structural Studies of Bacteriorhodopsin

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Authors

MGM. V. GoncharukMDM. A. DubinnyiONO. V. Nekrasova

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Overview

Randomized trial evaluates bicelles for high-resolution structural studies in bacteriorhodopsin, indicating promising results.

Key Points

  • This study aims to optimize protocols for isotopically labeled bacteriorhodopsin and evaluate membrane mimetics for NMR analysis.
  • BR expressed in E. coli BL21(DE3) as a Mistic fusion construct with isotopic labeling.
  • Purification using immobilized metal affinity and size-exclusion chromatography with various membrane mimetics.
  • NMR spectra recorded at 600-800 MHz to assess stability and signal characteristics.
  • Isotopically labeled BR yielded ~40-50 mg/L with bicelles showing best stability and spectral quality.
  • Rotational correlation times for bicelles at ~30 ns were significantly smaller than for nanodiscs at 44 ns.
  • Selective 15N-labeling enabled detection of over 300 spin systems, enhancing structural characterization.

Cite This Study

Goncharuk et al. (2026) studied this question.

synapsesocial.com/papers/6a06b74ce7dec685947aa4a9https://doi.org/10.1134/s1068162026601345
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