Computational-Aided Mutagenesis of Lipase CRL1 with Promoted Stability and Activity for Biosynthesis of Vitamin E Succinate

Key Points

• The aim is to enhance the stability and activity of lipase CRL1 through computational-aided mutagenesis to support vitamin E succinate biosynthesis.
• Generated mutant lipase variants using computational techniques.
• Evaluated the yield of vitamin E succinate at optimized conditions (60 °C for 12h).
• Mutant 6M achieved a 6.8-fold increase in yield compared to wild type.
• Yield for mutant 6M reached 77.8% under optimal conditions.