Incidence of Huanglongbing-Associated ‘CandidatusLiberibacter Asiaticus’ inDiaphorina citri(Hemiptera: Psyllidae) Collected from Plants for Sale in Florida

The Asian citrus psyllid, Diaphorina citri Kuwayama, was reported for the first time in Florida in June 1998, and huanglongbing (HLB, citrus greening), vectored by D. citri, was detected in Florida for the first time in Aug 2005. In Florida, the only known HLB pathogen is ‘Candidatus Liberibacter asiaticus’ (Las). After HLB was known to be established in Florida, the psyllid vectors found in regulatory samples from plants for sale were tested for the pathogen by real-time qPCR. Approximately 1,200 regulatory samples were tested between Aug 2005 and Aug 2009. Samples came from venues in 44 of Florida's 67 counties. Most of the samples came from citrus, but about 11% came from Murraya exotica, a popular ornamental plant and close relative of citrus. Approximately 9.7% of the psyllid samples tested were positive for Las. Numbers of samples and proportion of positive samples varied by year and by county.