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May 16, 2026Cardiovascular Research

High-resolution post-mortem CMR imaging clearly reveals intramyocardial haemorrhage in a rat model of acute myocardial infarction

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Why the study?

To establish a robust and reproducible diagnostic approach for detecting intramyocardial haemorrhage in a preclinical AMI model to facilitate translation to clinical practice.

Population

9 rats undergoing 45 minutes of myocardial ischemia

Comparison

Post-mortem high-resolution CMR vs in vivo CMR

Design

Preclinical animal study

Follow-up

3 hours after reperfusion

Key result

High-resolution post-mortem CMR imaging successfully visualized intramyocardial haemorrhage in a rat AMI model, whereas in vivo T2* mapping failed due to susceptibility and motion artifacts.

Authors

CKC T A KusterVRV P A Van ReijmersdalMWM Wiesmann

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Overview

Validates LGE CMR for confirming infarction in rat reperfusion models; leaves open translation to human IMH assessment.

Key Points

  • To establish a diagnostic approach for accurately detecting intramyocardial haemorrhage in a rat model of acute myocardial infarction post-reperfusion.
  • Rats (n=9) underwent 45 minutes of myocardial ischemia by occluding the left anterior descending coronary artery.
  • CMR imaging was performed post-reperfusion using T2* mapping to evaluate intramyocardial haemorrhage after 2-3 hours.
  • Macroscopic assessments with staining techniques (Thioflavin-S, Evans Blue, TTC) were conducted to validate findings.
  • Late gadolinium enhancement confirmed myocardial infarction in all rats after 3 hours of reperfusion.
  • Post-mortem T2* maps identified intramyocardial haemorrhage with reduced T2* values, while in vivo assessments were hindered by artifacts.
  • Corresponding staining and imaging verified the spatial alignment of intramyocardial haemorrhage with areas of necrosis.

Structured PICO

P
Population
Rats (n=9) subjected to 45 minutes of myocardial ischemia via occlusion of the left anterior descending coronary artery followed by reperfusion.
I
Intervention
High-resolution post-mortem CMR imaging (T2* mapping at 11.7T) of isolated retrogradely perfused hearts.
C
Comparator
In vivo CMR imaging (T2* mapping at 7T) after 2-3 hours of reperfusion.
O
Outcome
Visualization and detection of intramyocardial haemorrhage (IMH).surrogate

High-resolution post-mortem CMR provides accurate assessment of intramyocardial haemorrhage in small animal models, highlighting the limitations of in vivo T2* imaging due to artifacts.

Limitations

  • In vivo T2* imaging is susceptible to confounding factors such as motion and susceptibility artifacts.

Cite This Study

Kuster et al. (2026) studied Acute myocardial infarction (n=9). High-resolution post-mortem CMR imaging (T2* mapping) vs. In vivo CMR imaging (T2* mapping) was evaluated on Detection of intramyocardial haemorrhage (IMH). High-resolution post-mortem CMR imaging successfully visualized intramyocardial haemorrhage in a rat AMI model, whereas in vivo T2* mapping failed due to susceptibility and motion artifacts.

synapsesocial.com/papers/6a080985a487c87a6a40b610https://doi.org/10.1093/cvr/cvag092.048
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