Development of bioluminescent enzyme immunoassay for glycocholic acid based on singlechain variable fragment-nanoluciferase fusion | Synapse
May 16, 2026
Development of bioluminescent enzyme immunoassay for glycocholic acid based on singlechain variable fragment-nanoluciferase fusion
Key Points
The study aims to develop a reliable immunoassay for detecting glycocholic acid as a biomarker for hepatocellular carcinoma.
Development of a bioluminescent enzyme immunoassay using single-chain variable fragment-nanoluciferase fusion.
Evaluation of assay performance in detecting glycocholic acid levels.
The bioluminescent immunoassay demonstrated high sensitivity and specificity for glycocholic acid detection.
Significant correlation with levels in hepatocellular carcinoma patients was observed.
Abstract
As glycocholic acid (GCA) has been recognized as the biomarker for hepatocellular carcinoma (HCC), the GCA detection is crucial for the prevention, diagnosis, and treatment of HCC. With the advantages...