Development of bioluminescent enzyme immunoassay for glycocholic acid based on singlechain variable fragment-nanoluciferase fusion

Key Points

• The study aims to develop a reliable immunoassay for detecting glycocholic acid as a biomarker for hepatocellular carcinoma.
• Development of a bioluminescent enzyme immunoassay using single-chain variable fragment-nanoluciferase fusion.
• Evaluation of assay performance in detecting glycocholic acid levels.
• The bioluminescent immunoassay demonstrated high sensitivity and specificity for glycocholic acid detection.
• Significant correlation with levels in hepatocellular carcinoma patients was observed.