Development of bioluminescent enzyme immunoassay for glycocholic acid based on singlechain variable fragment-nanoluciferase fusion

Yunping Mu; Huajian Cai; Xindan Zhang; Mingxia Lin; Junhao Zeng; Yiyun Wang; Ziming Wu; Suqing Zhao; Xiping Cui

doi:10.1039/d6ay00731g

January 1, 2026Analytical Methods

Development of bioluminescent enzyme immunoassay for glycocholic acid based on singlechain variable fragment-nanoluciferase fusion

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Key Points

The study aims to develop a reliable immunoassay for detecting glycocholic acid as a biomarker for hepatocellular carcinoma.
Development of a bioluminescent enzyme immunoassay using single-chain variable fragment-nanoluciferase fusion.
Evaluation of assay performance in detecting glycocholic acid levels.
The bioluminescent immunoassay demonstrated high sensitivity and specificity for glycocholic acid detection.
Significant correlation with levels in hepatocellular carcinoma patients was observed.

Implication

Develops a bioluminescent assay for glycocholic acid, suggesting potential in hepatocellular carcinoma diagnosis.

Abstract

As glycocholic acid (GCA) has been recognized as the biomarker for hepatocellular carcinoma (HCC), the GCA detection is crucial for the prevention, diagnosis, and treatment of HCC. With the advantages...

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Cite This Study

Mu et al. (2026) studied this question.

synapsesocial.com/papers/6a0809bea487c87a6a40b9a5 https://doi.org/10.1039/d6ay00731g

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Development of bioluminescent enzyme immunoassay for glycocholic acid based on singlechain variable fragment-nanoluciferase fusion

Key Points

Implication

Abstract

Discussion

Cite This Study

Development of bioluminescent enzyme immunoassay for glycocholic acid based on singlechain variable fragment-nanoluciferase fusion

Key Points

Implication

Abstract

Discussion

Cite This Study

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