One-pot synthesis of fluorescent cap1 mRNAs using labelled trinucleotide m7G-cap analogs for real-time in vitro and in vivo mRNA tracking

Chou Yang; Chen Guo; Yiqian Zhou; Suyong Li; M Li; Yalin Xie; Yanhui Liu; Xinyu Lu; Lijun Zhang; Guoheng Mo; Wei Zhu; Jinlin Hou; Jiancun Zhang

doi:10.1039/d6ra00979d

January 1, 2026RSC AdvancesOpen Access

One-pot synthesis of fluorescent cap1 mRNAs using labelled trinucleotide m7G-cap analogs for real-time in vitro and in vivo mRNA tracking

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Key Points

The aim is to develop a simple method for creating mRNAs that can be tracked in cells and organisms using fluorescent markers.
One-pot synthesis of cap1 mRNAs using fluorophore-tagged trinucleotide analogs.
Application of synthesized mRNAs for both in vitro and in vivo studies.
Tracking of mRNA distribution in cellular imaging and animal models.
Successfully synthesized trackable mRNAs using cap1 analogs.
Fluorescent mRNAs showed effective tracking in cellular imaging.
Demonstrated broad distribution of mRNAs in whole animal studies.

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Implication

Demonstrates effective mRNA tracking in cellular and whole-animal studies using tagged analogs, suggesting broad applications.

Abstract

Fluorophore-tagged cap1 analogs provide a practical platform for the one-pot synthesis of trackable mRNAs for cellular imaging and whole-animal distribution studies.

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Yang et al. (2026) studied this question.

synapsesocial.com/papers/6a080acea487c87a6a40cd1f https://doi.org/10.1039/d6ra00979d

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One-pot synthesis of fluorescent cap1 mRNAs using labelled trinucleotide m7G-cap analogs for real-time in vitro and in vivo mRNA tracking

Key Points

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

One-pot synthesis of fluorescent cap1 mRNAs using labelled trinucleotide m7G-cap analogs for real-time in vitro and in vivo mRNA tracking

Key Points

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

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