The aim is to develop a simple method for creating mRNAs that can be tracked in cells and organisms using fluorescent markers.
One-pot synthesis of cap1 mRNAs using fluorophore-tagged trinucleotide analogs.
Application of synthesized mRNAs for both in vitro and in vivo studies.
Tracking of mRNA distribution in cellular imaging and animal models.
Successfully synthesized trackable mRNAs using cap1 analogs.
Fluorescent mRNAs showed effective tracking in cellular imaging.
Demonstrated broad distribution of mRNAs in whole animal studies.
Abstract
Fluorophore-tagged cap1 analogs provide a practical platform for the one-pot synthesis of trackable mRNAs for cellular imaging and whole-animal distribution studies.