Key points are not available for this paper at this time.
parameters for 11 enzyme/substrate pairs. We showcase how this method can be employed for assaying other UGT phenomena, specifically hydrolysis of the glycosyl donor and inactivation upon enzyme dilution. The reverse glycosylation reaction with 2-chloro-4-nitrophenyl-β-d-glycosides can be catalyzed by all 33 assayed UGTs, and therefore the methodology qualifies as a cheap generic tool for high-throughput assays of these enzymes. Moreover, we provide a script for automatic determination of rates and kinetics and present an acceptor data set consisting of 2280 UGT/acceptor pairs.
Langhorn et al. (Fri,) studied this question.