Singlet oxygen ( 1 O 2 )-catalyzed oxidation of water leads to a variety of oxidants (e.g. ozone, O 3 ) in host-parasite relationships as well. It can be detected indirectly in TLC or OPLC zones by use of the simple BioArena system and O 3 -eliminating molecules (e.g. d -limonene and indigo carmine) in the culture medium. It follows from these new findings that not only formaldehyde but also O 3 and related bioreactive compounds may play a crucial role in the mechanism of antibacterial activity of antibiotic-like compounds. The toxic potential of a molecule, however, originates from the ratio of the oxidants produced in the chromatographic spots.
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Tyihák et al. (2008) studied this question.
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