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August 1, 1992Journal of Biological ChemistryOpen Access

The 3'-flanking sequence of the bovine growth hormone gene contains novel elements required for efficient and accurate polyadenylation.

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Authors

EGEdward C. GoodwinFRFritz Rottman

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Overview

Randomized trial determines essential polyadenylation elements in bovine growth hormone gene, suggesting novel mechanisms of efficiency.

Key Points

  • This research aims to identify the sequence requirements critical for efficient and accurate polyadenylation of the bovine growth hormone gene.
  • Conducted quantitative S1 nuclease analysis of transcripts from various bGH 3' deletions and block mutations.
  • Used COS-1 cells for transient transfection to assess polyadenylation efficiency.
  • Analyzed effects of mutations on GU and U-rich sequences in the 3'-flanking region.
  • Mutation of the putative GU efficiency element resulted in only a marginal decrease in polyadenylation efficiency.
  • Other sequences lacking GU or U-rich motifs demonstrated similar effects, indicating a diffuse efficiency element.
  • Identified a specific region 18 to 27 nucleotides downstream of the cleavage site necessary for accurate positioning.

Cite This Study

Goodwin et al. (1992) studied this question.

synapsesocial.com/papers/6a08ee83aa03afa536e4b547https://doi.org/10.1016/s0021-9258(18)42005-4
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