ZSF1-obese rats, a model of HFpEF, exhibited higher resting and steady-state mitochondrial calcium concentrations and lower mitochondrial respiration compared to ZSF1-lean controls.
Abstract Aim Calcium ions play a pivotal role in matching energy supply and demand in cardiac muscle. Mitochondrial calcium concentration is lower in animal models of heart failure with reduced ejection fraction (HFrEF), but limited information is available about mitochondrial calcium handling in heart failure with preserved ejection fraction (HFpEF). Methods We assessed mitochondrial Ca 2+ handling in intact cardiomyocytes from Zucker/fatty Spontaneously hypertensive F1 hybrid (ZSF1)‐lean (control) and ZSF1‐obese rats, a metabolic risk‐related model of HFpEF. A mitochondrially targeted Ca 2+ indicator (MitoCam) was expressed in cultured adult rat cardiomyocytes. Cytosolic and mitochondrial Ca 2+ transients were measured at different stimulation frequencies. Mitochondrial respiration and swelling, and expression of key proteins were determined ex vivo. Results At rest, mitochondrial Ca 2+ concentration in ZSF1‐obese was larger than in ZSF1‐lean. The diastolic and systolic mitochondrial Ca 2+ concentrations increased with stimulation frequency, but the steady‐state levels were larger in ZSF1‐obese. The half‐widths of the contractile responses, the resting cytosolic Ca 2+ concentration and the decay half‐times of the cytosolic Ca 2+ transients were higher in ZSF1‐obese, likely because of a lower SERCA2a/phospholamban ratio. Mitochondrial respiration was lower, particularly with nicotinamide adenine dinucleotide (NADH) (complex I) substrates, and mitochondrial swelling was larger in ZSF1‐obese. Conclusion The free mitochondrial calcium concentration is higher in HFpEF owing to alterations in mitochondrial and cytosolic Ca 2+ handling. This coupling between cytosolic and mitochondrial Ca 2+ levels may compensate for myocardial ATP supply in vivo under conditions of mild mitochondrial dysfunction. However, if mitochondrial Ca 2+ concentration is sustainedly increased, it might trigger mitochondrial permeability transition pore opening.
Miranda‐Silva et al. (Sat,) conducted a other in Heart failure with preserved ejection fraction (HFpEF). ZSF1-obese rat model vs. ZSF1-lean rats (control) was evaluated on Mitochondrial and cytosolic Ca2+ handling, respiration, and swelling. ZSF1-obese rats, a model of HFpEF, exhibited higher resting and steady-state mitochondrial calcium concentrations and lower mitochondrial respiration compared to ZSF1-lean controls.