⌘+K

Pulse Explore Communities Researchers Journals

Home Communities Explore

May 15, 2026ACS Synthetic Biology

CRISPR/Cas9 Recombineering with Bacteriophage Recombinases Enables Efficient and Scarless Genome Editing in Cupriavidus necator H16

Yuzhong Liu Caidie Wang Yiyuan Tian J J Li Zeyu Wu Pengxia Wang Wei Xiong

View at Publisher

Ask AI

Bookmark

Share

Key Points

The research aims to develop an efficient method for genome editing in Cupriavidus necator using CRISPR/Cas9 and bacteriophage recombinases.
Utilized CRISPR/Cas9 technology for genome editing
Employed bacteriophage recombinases to facilitate recombineering
Focused on strain development for metabolic engineering applications.
Achieved efficient genome editing with high precision
Demonstrated scarless modifications in the Cupriavidus necator genome
Showed potential for improved C1 metabolic pathways in industrial applications.

No abstract yet

We only have this paper's title and publication details.

Read it at the source→

Implication

Demonstrates efficient genome editing in Cupriavidus necator, suggesting enhanced metabolic engineering.

Abstract

strain development for C1 metabolic engineering and industrial applications.

Discussion

No takes yet. Share an insight, caveat, or question.

Cite This Study

Liu et al. (2026) studied this question.

synapsesocial.com/papers/6a0aac6d5ba8ef6d83b6fdde https://doi.org/10.1021/acssynbio.6c00055

View at Publisher

Ask AI

Bookmark

Share

Keep reading

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

1Establishing CRISPRi for Programmable Gene Repression and Genome Evolution in <i>Cupriavidus necator</i>2024 · 24 citations
2CRISPR/Cas9 and Cytidine Base-Editing Systems for Efficient Genome Engineering in Oleaginous <i>Rhodococcus</i>Original Research · 2026 · 2 citations
3Development of a recombineering system for the acetogen Eubacterium limosum with Cas9 counterselection for markerless genome engineering2024 · 1 citations
4Establishment of the CRISPR-Cpf1 gene editing system in Bacillus licheniformis and multiplexed gene knockout2024 · 11 citations
5A Lambda Red Recombinase‐Assisted CRISPR /Cas9 System for Efficient and Precise Genome Editing in Agrobacterium tumefaciensOriginal Research · 2026

More related papers

An inducer-independent, single-plasmid CRISPR-Cas9 system for genome editing in Bacillus species2026
Genetic manipulation of Bacteriophage T4 utilizing the CRISPR-Cas13b system2024
A Minimal and Portable CRISPR Platform Based on Bifidobacterial Cas9 Enables Genome Editing in <i>E. coli</i> Nissle 19172026

More from ACS Synthetic Biology

Yeast Metabolic Engineering for Carotenoid Biosynthesis: Hosts, Pathways, Strategies, and PerspectivesFri,
In Vivo Continuous Evolution Systems: Overview, Principles and ApplicationsFri,