The ureides allantoin and allantoic acid are important transport compounds of fixed nitrogen from soybean (Glycine max L. Merr.) nodules to the plant tops. The objective of this research was to develop a colorimetric method for determination of ureides in soybean tissues. The 2,3-butanedione monoxime-thiosemicarbazide (BDM-TSC) method for urea was adapted for ureide analysis by adding an alkaline hydrolysis step and converting allantoin to allantoate before determination as urea. Water extracts of soybean tissue samples containing a wide range of ureide concentrations were analyzed by the proposed method and two alternative procedures. The alternative procedures included a volumetric method based on urease and a colorimetric method for ureide-derived glyoxylic acid. The proposed method was highly correlated (r = 0.99) to the two alternative methods. The proposed method was easier to perform than the two alternative methods and should be adaptable for use by microplate methods.
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Goos et al. (2014) studied this question.
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