What question did this study set out to answer?

To establish a higher-order multiplexed mass spectrometry method that enhances throughput and statistical power for profiling dynamic cellular signaling events.

March 27, 2012Science SignalingOpen Access

Hyperplexing: A Method for Higher-Order Multiplexed Quantitative Proteomics Provides a Map of the Dynamic Response to Rapamycin in Yeast

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To establish a higher-order multiplexed mass spectrometry method that enhances throughput and statistical power for profiling dynamic cellular signaling events.
Combined triplex metabolic labeling with six-plex isobaric mass tags to create an 18-sample simultaneous quantification platform.
Profiled biological triplicates across a six-point time course in yeast stimulated with rapamycin.
Simultaneously quantified protein abundance across 18 samples, overcoming throughput limits of standard mass spectrometry workflows.
Mapped dynamic temporal abundance profiles for thousands of yeast proteins responding to TOR pathway inhibition.

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Implication

Experimental study demonstrates 18-sample multiplexed quantitative proteomics in yeast, highlighting dynamic temporal regulation across the TOR signaling pathway.

Cite This Study

Dephoure et al. (2012) studied this question.

synapsesocial.com/papers/6a0b28d76f9280a32b5d366d https://doi.org/10.1126/scisignal.2002548

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Implication

Experimental study demonstrates 18-sample multiplexed quantitative proteomics in yeast, highlighting dynamic temporal regulation across the TOR signaling pathway.

Hyperplexing: A Method for Higher-Order Multiplexed Quantitative Proteomics Provides a Map of the Dynamic Response to Rapamycin in Yeast

Key Points

Discussion

Implication

Cite This Study

Hyperplexing: A Method for Higher-Order Multiplexed Quantitative Proteomics Provides a Map of the Dynamic Response to Rapamycin in Yeast

Key Points

Discussion

Implication

Cite This Study

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