A nonproteolytic toxic fraction was found present in agar extracts of Pseudomonas aeruginosa grown on tryptone-glucose for 72 hr. The toxic fraction could not be separated by chemical means from a protease which exhibits elastase activity. However, the moieties were separable by use of column chromatography employing DEAE-Sephadex A-50. Mouse lethality was obtained by the intraperitoneal and intravenous routes. The mouse LD50 values were 237 and 105 μg of protein, respectively. The intranasal and subcutaneous routes were not lethal within the concentrations tested. No gross pathological changes were noted with the exception of occasional petechial lung hemorrhage.
No takes yet. Share an insight, caveat, or question.
Meinke et al. (1970) studied this question.