Combined treatment with metoprolol and atractylenolide I improved LVEF, LVFS, and NO, and decreased LVMI, ET-1, TNF-α, IL-6, LDH, CK-MB, and CK in rats with acute myocardial infarction.
RCT (n=50)
Randomly divided
Does combined metoprolol and atractylenolide I improve cardiac function and reduce infarct size in a rat model of acute myocardial infarction?
Combined metoprolol and atractylenolide I provides synergistic cardioprotection in a rat model of acute myocardial infarction via the SIRT3/β-catenin/PPAR-γ pathway.
Herein, we examined the protective effect of metoprolol combined with atractylenolide I (Atr I) in acute myocardial infarction (AMI) by regulating the SIRT3 (silent information regulator 3)/β-catenin/peroxisome proliferator-activated receptor gamma (PPAR-γ) signaling pathway. Briefly, 50 rats were randomly divided into the sham operation, model, metoprolol, Atr I, and combination metoprolol with Atr I groups (combined treatment group). The AMI model was established by ligating the left anterior descending coronary artery. After treatment, infarct size, histopathological changes, and cell apoptosis were examined using 2,3,5-triphenyltetrazolium chloride staining, hematoxylin-eosin staining, and the TUNEL assay. The left ventricular ejection fraction (LVEF), left ventricular fraction shortening (LVFS), and left ventricular mass index (LVMI) were detected by echocardiography. Endothelin-1 (ET-1), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6) levels were detected using enzyme-linked immunosorbent assays. Furthermore, we measured lactate dehydrogenase (LDH), creatine kinase (CK) isoenzyme (CK-MB), and CK levels. Western blotting was performed to determine the expression of SIRT3, β-catenin, and PPAR-γ. Herein, the combined treatment group exhibited increased levels of LVEF, LVFS, and NO, whereas LVMI, ET-1, TNF-α, IL-6, LDH, CK-MB, and CK levels were decreased. Importantly, the underlying mechanism may afford protection against AMI by increasing the expression levels of SIRT3, β-catenin, and PPAR-γ.
Zhou et al. (Sun,) conducted a rct in Acute Myocardial Infarction (AMI) (n=50). Metoprolol combined with atractylenolide I vs. Sham operation, model, metoprolol alone, and atractylenolide I alone was evaluated on LVEF, LVFS, LVMI, infarct size, cell apoptosis, and biomarker levels (ET-1, NO, TNF-α, IL-6, LDH, CK-MB, CK). Combined treatment with metoprolol and atractylenolide I improved LVEF, LVFS, and NO, and decreased LVMI, ET-1, TNF-α, IL-6, LDH, CK-MB, and CK in rats with acute myocardial infarction.
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