Mouse colorectal cancer organoids: Lessons from syngeneic and orthotopic transplantation systems

This is the authors' abstract. We don't add key points for this paper.

Colorectal cancer (CRC) organoids provide more accurate and tissue-relevant models compared to conventional two-dimensional cultured cell cultures. Mouse CRC organoids, in particular, offer unique advantages over their human counterparts, as they can be transplanted into immunocompetent mice. These syngeneic transplantation models create a robust system for studying cancer biology in the immunocompetent tumor microenvironment (TME). This article discusses the development and applications of these organoid systems, emphasizing their capacity to faithfully recapitulate in vivo tumor progression, metastasis, and the immune landscape. • Cancer organoids are essential models for studying tumor biology and tumor microenvironment in colorectal cancer. • Mouse colorectal cancer organoids can be transplanted into immunocompetent mice. • Syngeneic and orthotopic transplantation enables full recapitulation of immune system providing critical insights into the comprehensive study of tumor biology and therapeutic strategies.