Key result
Simultaneous in situ hybridization using biotinylated and 35S-labeled riboprobes successfully detected two mRNAs in the same cell, though radiolabeled probe sensitivity was reduced by about 40%.
Simultaneous in situ hybridization using biotinylated and 35S-labeled riboprobes allows detection of two mRNAs in the same cell, albeit with a 40% reduction in radiolabeled probe sensitivity.
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May enable dual mRNA co-detection in research; leaves open clinical translation given sensitivity loss.
Ozden et al. (1990) studied Theiler's virus infection (in vitro). Simultaneous in situ hybridization with biotinylated and 35S-labeled riboprobes was evaluated on Simultaneous detection of two mRNAs. Simultaneous in situ hybridization using biotinylated and 35S-labeled riboprobes successfully detected two mRNAs in the same cell, though radiolabeled probe sensitivity was reduced by about 40%.
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