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August 1, 1978Journal of Histochemistry & Cytochemistry332 citationsOpen Access

Acetylation of chromosome squashes of Drosophila melanogaster decreases the background in autoradiographs from hybridization with 125I-labeled RNA.

SHShizu HayashiIGI.C. GillamADA. D. Delaney

Key Points

  • This research aims to evaluate the impact of acetylation on autoradiography results in Drosophila chromosomes.
  • Prepared chromosomes from larval salivary glands of Drosophila melanogaster were hybridized with [125I]-labeled RNA.
  • Autoradiography was conducted to assess the binding of radioactivity to chromosome regions.
  • Acetylation of chromosome preparations was performed prior to hybridization to reduce background noise.
  • Acetylation significantly decreased background radioactivity in autoradiographs, revealing clearer chromosomal labeling.
  • Without acetylation, radioactivity masked specific sites, complicating the detection of labeled chromosomes.

Abstract

DNA in prepared chromosomes from the larval salivary glands of Drosophila melanogaster was hybridized with 125I-labeled 5S and tRNA from the same organism. Autoradiography revealed that radioactivity was frequently bound to all regions of the slides, masking labeling of the chromosomes. Acetylation of the preparations before hybridization prevented the formation of this background and revealed the specific chromosomal sites.

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Cite This Study

Hayashi et al. (1978) studied this question.

synapsesocial.com/papers/6a0cc7259d761985b14a4836https://doi.org/10.1177/26.8.99471
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