Collagen-Coated Cellulose Sponge: Three-Dimensional Matrix for Tissue Culture of Walker Tumor 256

Three-dimensional growth of large populations of cells in vitro has been observed in the interstices of a matrix consisting of collagen-coated cellulose sponge. The growth of Walker tumor 256 in this composite matrix is compared with that found in a matrix composed of either cellulose sponge alone or collagen sponge alone. The composite matrix is superior to either one. Collagen-coated cellulose sponge may provide a simple tool for the study of social interaction of cells in the formation of organized elementary tissue structures.