Key result
Reovirus-infected cardiac endothelial cells recruit cytotoxic T cells and undergo pyroptosis in neonatal mice.
Why the study?
A significant fraction of sudden death in children and young adults is caused by viral myocarditis, an inflammatory heart disease.
Single-cell and spatial transcriptomics reveal that inflamed endothelial cells recruit cytotoxic T cells and undergo pyroptosis during reovirus-induced myocarditis in neonatal mice.
Hypothesis-generating for endothelial pyroptosis in neonatal viral myocarditis; leaves open human relevance and therapeutic targeting.
A significant fraction of sudden death in children and young adults is due to viral myocarditis, an inflammatory disease of the heart. In this study, by using integrated single-cell and spatial transcriptomics, we created a high-resolution, spatially resolved transcriptome map of reovirus-induced myocarditis in neonatal mouse hearts. We assayed hearts collected at three timepoints after infection and studied the temporal, spatial and cellular heterogeneity of host-virus interactions. We further assayed the intestine, the primary site of reovirus infection, to establish a full chronology of molecular events that ultimately lead to myocarditis. We found that inflamed endothelial cells recruit cytotoxic T cells and undergo pyroptosis in the myocarditic tissue. Analyses of spatially restricted gene expression in myocarditic regions and the border zone identified immune-mediated cell-type-specific injury and stress responses. Overall, we observed a complex network of cellular phenotypes and spatially restricted cell-cell interactions associated with reovirus-induced myocarditis in neonatal mice.
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Mantri et al. (2022) studied Viral myocarditis. Reovirus T1L infection vs. Mock infection was evaluated on Spatiotemporal transcriptomic mapping of host-virus interactions. Integrated single-cell and spatial transcriptomics in a neonatal mouse model revealed that reovirus-infected cardiac endothelial cells recruit cytotoxic T cells and undergo pyroptosis.
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