Key result
Downregulated circSamd4a is linked to vascular calcification with a diagnostic AUC of 0.81.
Why the study?
Vascular calcification is commonly associated with cardiovascular diseases, prompting the study to analyze circRNA expression and evaluate biomarkers for diagnosing vascular calcification.
Does plasma circSamd4a level predict the presence of coronary artery calcification in hospitalized patients?
Case-Control (n=71)
Does plasma circSamd4a level predict the presence of coronary artery calcification in hospitalized patients?
CircSamd4a is significantly downregulated in vascular calcification across cellular, animal, and human models, and shows strong potential as a novel diagnostic biomarker for coronary artery calcification.
BACKGROUND: Vascular calcification (VC) is usually associated with cardiovascular diseases (CVDs), which are one of the main causes of mortality in the world. This study aimed to analyze the expression of circular RNAs (circRNAs) in patients with VC and to evaluate biomarkers for the diagnosis of VC. METHODS: Calcified human aortic endothelial cells (HAECs) and the calcification in mouse aorta were detected by qRT-PCR. Subsequently, this was verified in the plasma of patients with coronary artery calcification (CAC). The plasma of 40 patients in the control group and 31 patients in the calcified group were detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR) to detect the level of circSamd4a in the blood. The diagnostic value was evaluated by logistic regression analysis and the working characteristics of subjects. RESULTS: In the HAECs, the qRT-PCR showed a significant decrease in the level of circSamd4a expression in the calcification group compared to the control group (p < 0.05). The calcified mouse aorta showed the same trend for circSamd4a expression, wherein the difference was statistically significant (p < 0.05); the expression of circSamd4a was significantly downregulated in the plasma of patients with VC (p < 0.01). The receiver operating characteristic (ROC) curves of circSamd4a in patients with VC and control group showed that the area under the curve (AUC) was 0.81 (95% CI: 0.707-0.913; p < 0.001). CONCLUSION: CircSamd4a showed a stable downward trend in different specimens, and had significant advantages as a biomarker for diagnosis of VC.
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Zhou et al. (2021) conducted a case-control in Vascular calcification (n=71). circSamd4a biomarker vs. Control group was evaluated on Diagnostic value of circSamd4a for vascular calcification (AUC 0.81, 95% CI 0.707-0.913, p=<0.001). CircSamd4a expression was significantly downregulated in patients with vascular calcification and demonstrated high diagnostic value (AUC 0.81; 95% CI 0.707-0.913; p<0.001).
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