Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
October 1, 2011AngiogenesisOpen Access

Ginsenoside-Rg1 mediates a hypoxia-independent upregulation of hypoxia-inducible factor-1α to promote angiogenesis

View Full Paper
Ask AI
Bookmark
Share

Key result

Ginsenoside-Rg1 stimulated hypoxia-inducible factor-1α accumulation and promoted angiogenesis in human umbilical vein endothelial cells via the PI3K/Akt and p70 S6K pathways under normal oxygen conditions.

Why the study?

Does Ginsenoside-Rg1 promote angiogenesis via HIF-1α upregulation in human umbilical vein endothelial cells?

Population

Human umbilical vein endothelial cells (HUVECs) (fifth to eighth passages)

Comparison

Ginsenoside-Rg1 (150 nM) vs Untreated control

Design

Preclinical

Authors

KLKar-Wah LeungUniversity of Hong KongHNHoi Man NgKiang Wu HospitalMTMaggie K. S. TangHong Kong Science and Technology Parks Corporation

Discussion

Loading...

Member takes

Implication

Hypothesis-generating for ginsenoside-Rg1 in normoxic angiogenesis; leaves open clinical translation pending in vivo studies.

Key Points

  • To elucidate the molecular mechanism through which the Panax ginseng derivative ginsenoside-Rg1 induces hypoxia-inducible factor-1α and drives angiogenesis under normal oxygen conditions.
  • Cultured human umbilical vein endothelial cells were treated with ginsenoside-Rg1 under normoxic conditions to evaluate HIF-1α mRNA and protein synthesis.
  • Involved signaling cascades and downstream gene activation were dissected using the pathway inhibitors LY294002 (PI3K/Akt) and rapamycin (p70(S6K)), alongside RNA interference-mediated knockdown of HIF-1α during tube formation assays.
  • Ginsenoside-Rg1 stimulated HIF-1α protein translation and subsequent vascular endothelial growth factor (VEGF) expression under normal cellular oxygen conditions without altering steady-state HIF-1α mRNA levels.
  • Activation was mediated by constitutive phosphorylation of PI3K/Akt and p70(S6K), whereas chemical inhibition of this axis or siRNA knockdown of HIF-1α suppressed VEGF induction and endothelial tube formation.

Structured PICO

Does Ginsenoside-Rg1 promote angiogenesis via HIF-1α upregulation in human umbilical vein endothelial cells?

P
Population
Human umbilical vein endothelial cells (HUVECs) (fifth to eighth passages)
I
Intervention
Ginsenoside-Rg1 (150 nM)
C
Comparator
Untreated control
O
Outcome
HIF-1α protein accumulation and angiogenic tube formationsurrogate

Main Result

p-value: p=<0.05

Ginsenoside-Rg1 promotes angiogenesis through a hypoxia-independent, PI3K/Akt/p70S6K-mediated upregulation of HIF-1α protein synthesis.

Limitations

  • In vitro study design
  • In vivo mechanisms and clinical efficacy remain to be fully elucidated

Cite This Study

Leung et al. (2011) studied Angiogenesis (in vitro). Ginsenoside-Rg1 vs. Untreated control was evaluated on HIF-1α protein accumulation and angiogenic tube formation (p=<0.05). Ginsenoside-Rg1 stimulated hypoxia-inducible factor-1α accumulation and promoted angiogenesis in human umbilical vein endothelial cells via the PI3K/Akt and p70 S6K pathways under normal oxygen conditions.

synapsesocial.com/papers/6a0da7c2d8df3832a209b4cfhttps://doi.org/10.1007/s10456-011-9235-z
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 3 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Ginsenoside-Rg1 induces angiogenesis via non-genomic crosstalk of glucocorticoid receptor and fibroblast growth factor receptor-12010 · 61 citations
  2. 2Purification and Characterization of Hypoxia-inducible Factor 11995 · 2,095 citations
  3. 3Current perspectives on Chinese medicines and dietary supplements in China, Japan and the United States2020 · 28 citations