Key result
Synthetic rat atrial natriuretic factor stimulated cyclic GMP formation by up to several hundred-fold in normal and malignant osteoblasts, which possess specific high-affinity ANF binding sites.
Synthetic rat atrial natriuretic factor stimulates cyclic GMP formation and binds to specific high-affinity receptors in both normal and malignant osteoblasts.
ANF responsiveness in osteoblasts remains preclinical; leaves open roles in bone physiology and malignancy.
Synthetic rat atrial natriuretic factor (Ile‐ANF‐26) stimulated cyclic GMP formation by up to several hundred‐fold in osteoblast‐rich cultures from newborn rat calvaria and in clonal osteogenic sarcoma cells (UMR 106‐01) which are phenotypically osteoblast. ANF had no effect on the cyclic AMP response to parathyroid hormone in the same cells. Specific, high‐affinity binding sites for ANF were identified in both cell types, with K d and receptor numbers in normal osteoblasts of 1.2 ± 0.1 × 10 −10 M and 42 ± 4 × 10 3 per cell, and in UMR 106‐01 cells of 1.4 ± 0.1 × 10 −10 M and 22 ± 4 × 10 3 per cell.
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Fletcher et al. (1986) studied Normal and malignant osteoblasts. Synthetic rat atrial natriuretic factor (Ile-ANF-26) was evaluated on Cyclic GMP formation and specific binding sites. Synthetic rat atrial natriuretic factor stimulated cyclic GMP formation by up to several hundred-fold in normal and malignant osteoblasts, which possess specific high-affinity ANF binding sites.
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