Key result
Membrane depolarization with 80 mM K+ increases T2 relaxation time by ~1 ms in rats.
Why the study?
Existing methods for measuring membrane potential are often invasive or have limited recording depth, whereas MRI offers noninvasive imaging with desirable spatial resolution over large areas.
Does manipulation of membrane potential alter magnetic resonance parameters (T2 and PSR) in cultured cells and in vivo rat models?
Does manipulation of membrane potential alter magnetic resonance parameters (T2 and PSR) in cultured cells and in vivo rat models?
Effect estimate: ΔT2 difference 0.918 ms
Absolute Event Rate: 1.1% vs 0.181%
p-value: p=0.00172
MRI parameters such as T2 relaxation time and pool size ratio can detect changes in cellular membrane potential, offering a potential noninvasive approach for functional imaging.
Membrane potential plays a crucial role in various cellular functions. However, existing techniques for measuring membrane potential are often invasive or have limited recording depth. In contrast, magnetic resonance imaging (MRI) offers noninvasive imaging with desirable spatial resolution over large areas. This study investigates the feasibility of utilizing MRI to detect membrane potential changes by measuring magnetic resonance parameters while manipulating membrane potential in cultured cells and in vivo rat models. Our findings reveal that depolarization (or hyperpolarization) of the membrane potential increases (or decreases) the T2 relaxation time, while the ratio of bound to free water proton shows the opposite trend. These findings also suggest a pioneering approach to noninvasively detect changes in membrane potential using MRI.
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Min et al. (2024) studied Membrane potential changes (n=12). Membrane potential depolarization via high K+ concentration vs. Baseline aCSF (3 mM K+) was evaluated on Change in T2 relaxation time (ΔT2) at 24 minutes (ΔT2 difference 0.918 ms, p=0.00172). Depolarization of the membrane potential using 80 mM K+ significantly increased T2 relaxation time by 1.10 ms compared to 0.181 ms in controls in an in vivo rat model.
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