A Perspective on How Fibrinaloid Microclots and Platelet Pathology May be Applied in Clinical Investigations

Figures

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  Fig. 1. Visualizing various factors that influence disease to the understand the phenomena of the disease due to the response to an external stimulus 44 such as an infection. Figure created by authors using Biorender.com.

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  Fig. 2. Platelet receptors and interactions with cells and proteins in circulation. Figure created by authors using Biorender.com.

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  Fig. 3. Fluorescence microscopy imaging examples of the different stages of platelet activation and spreading that was used to score platelet activation in long COVID patients. After centrifuging freshly collected samples, the hematocrit fraction of each sample was retained and incubated for 30 minutes at room temperature with the two fluorescent markers, CD62P (PE-conjugated) (platelet surface P-selectin) (IM1759U, Beckman Coulter, Brea, CA) and PAC-1 (FITC-conjugated) (340507, BD Biosciences, San Jose, CA). 10 mL of each exposed sample was placed on microscope slide and viewed using a 63x oil objective. Stage 1, with minimally activated platelets, seen as small round platelets with a few pseudopodia, seen as healthy/control platelets that progresses to Stage 4, with egg-shaped platelets, indicative of spreading and the beginning of clumping (with permission from the CC-BY publication 28 ).

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  Fig. 4. Fluorescence microscopy showing microclots in platelet poor plasma (PPP) with representative examples of the different stages of different stages of microclot formation. Stored or freshly prepared platelet poor plasma samples were exposed to Thioflavin T (ThT), a fluorogenic dye that binds to amyloid protein. 15 A final concentration of 0.005 mM was used (Sigma-Aldrich, St. Louis, MO). Plasma was exposed for 30 minutes (protected from light) at room temperature, whereafter 3 µL stained PPP was placed on a glass slide and covered with a coverslip. Stage 1 shows minimal microclot formation in healthy/control PPP which progresses to the presence of the severe microclotting Stage 4. Bottom row represents examples of stage 4 microclots using ( A ) bright-field microscopy, ( B ) fluorescence microscopy, and ( C ) an overlay of fluorescence and bright-field microscopy (with permission from the CC-BY publication 28 ).

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  Fig. 5. Protein–protein interactions may result in protein misfolding and have been shown to cause amyloidogenic changes to all kinds of proteins. This is illustrated in the upper part for the Prion protein PrP. The lower part shows electron micrographs of fibrin clots. Adapted in part from the CC-BY publications. 15 17 64 We also note that the spike protein is itself amyloidogenic. 172 Figure created by authors using Biorender.com.

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  Fig. 6. Comparison of microclot area in healthy participants, and participants with Type 2 diabetes and acute COVID-19. (Raw data reanalyzed, and available in 26 ).

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  Fig. 7. ( A to F ) Whole blood scanning electron microscopy of micrograph data previously collected in published studies. ( A ) A few plasma deposits are discernible within the blood sample of a healthy participant 173 ; ( B ) systemic lupus erythematosus 174 ; ( C and D ) acute COVID-19 during the initial wave in 2020 30 ; ( E ) rheumatoid Arthritis 175 ; ( F ) Alzheimer's-type disease. 176