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May 15, 2025PLoS ONEOpen Access

Network pharmacology and experimental validation to investigate the mechanism of action of Zhilong Huoxue Tongyu capsule in the prevention and treatment of diabetic cardiomyopathy

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Why the study?

Diabetic cardiomyopathy has a multifaceted pathogenesis, and the mechanism of action of Zhilong Huoxue Tongyu Capsule in this condition was not fully understood.

Does Zhilong Huoxue Tongyu Capsule mitigate myocardial fibrosis and alter protein expression in a mouse model of diabetic cardiomyopathy?

Population

DCM model mice

Comparison

Zhilong Huoxue Tongyu capsule vs SB203580 vs rosiglitazone vs physiological saline control

Design

Animal experimental study with network pharmacology analysis

Follow-up

6 weeks

Key result

Zhilong Huoxue Tongyu Capsule significantly mitigated myocardial fibrosis and down-regulated p-P38MAPK, TNF-α, α-SMA, and Collagen-I proteins in a mouse model of diabetic cardiomyopathy.

Authors

FYFang YangGLGang LuoMLMengnan Liu

Discussion

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Overview

Hypothesis-generating in diabetic cardiomyopathy mice; should not change clinical practice pending human data.

Structured PICO

Does Zhilong Huoxue Tongyu Capsule mitigate myocardial fibrosis and alter protein expression in a mouse model of diabetic cardiomyopathy?

P
Population
Diabetic cardiomyopathy (DCM) model mice
I
Intervention
Zhilong Huoxue Tongyu Capsule (ZL) 6.24g/kg/d via gavage for 6 weeks
C
Comparator
Equal volume of physiological saline by gavage (DCM group and control group), SB203580 1 mg/kg/d via intraperitoneal injection, and rosiglitazone 4 mg/kg/d via gavage
O
Outcome
Myocardial fibrosis and expression of p-P38MAPK, TNF-α, α-SMA, and Collagen-I proteins in myocardial tissuesurrogate

Zhilong Huoxue Tongyu Capsule ameliorates diabetic cardiomyopathy in mice, likely by modulating the P38MAPK signaling pathway.

Cite This Study

Yang et al. (2025) studied Diabetic cardiomyopathy. Zhilong Huoxue Tongyu Capsule (ZL) vs. Physiological saline, SB203580, and rosiglitazone was evaluated on Myocardial fibrosis and expression of p-P38MAPK, TNF-α, α-SMA, and Collagen-I proteins. Zhilong Huoxue Tongyu Capsule significantly mitigated myocardial fibrosis and down-regulated p-P38MAPK, TNF-α, α-SMA, and Collagen-I proteins in a mouse model of diabetic cardiomyopathy.

synapsesocial.com/papers/6a0e98d920dec68b0dc841a2https://doi.org/10.1371/journal.pone.0323745
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